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  • Blooms Syndrome Antibodies

Invitrogen

Blooms Syndrome Polyclonal Antibody

Advanced Verification
2 Published Figures
5 References
View all (24) Blooms Syndrome antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Blooms Syndrome Polyclonal Antibody

  • Antibody Testing Data (7)
  • Published Figures (2)
  • Advanced Verification (1)
Blooms Syndrome Antibody in Immunocytochemistry (ICC/IF)
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Blooms Syndrome Antibody in Immunocytochemistry (ICC/IF)
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Blooms Syndrome Antibody (PA5-27384) in ICC/IF

Immunofluorescence analysis of Blooms Syndrome was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Blooms Syndrome Polyclonal Antibody (Product # PA5-27384) at 5µg/mL in 0.1% BSA, incubated at 4 degree Cel... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Blooms Syndrome Antibody in Immunocytochemistry (ICC/IF)
Blooms Syndrome Antibody in Immunocytochemistry (ICC/IF)
Blooms Syndrome Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Blooms Syndrome Antibody in Western Blot (WB)
Blooms Syndrome Antibody in Western Blot (WB)
Blooms Syndrome Antibody in Western Blot (WB)
Blooms Syndrome Antibody in Western Blot (WB)
Blooms Syndrome Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
Blooms Syndrome Antibody in Immunocytochemistry (ICC/IF)
Blooms Syndrome Antibody
Blooms Syndrome Polyclonal Antibody

Product Details

PA5-27384

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human

Published species

Fruit fly

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BLM. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.34 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544860

Product Specific Information

Recommended positive controls: Jurkat, Raji, K562, NCI-H929, NIH-3T3, JC, BCL-1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Bloom syndrome protein; Bloom syndrome, RecQ helicase-like; DNA 3'-5' helicase BLM; DNA helicase, RecQ-like type 2; MGC126616; MGC131618; MGC131620; RecQ protein-like 3; RecQ-like DNA helicase BLM; RecQ2

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Gene Aliases: BLM; BS; RECQ2; RECQL2; RECQL3

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UniProt ID: (Human) P54132

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Entrez Gene ID: (Human) 641

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Function(s)
bubble DNA binding p53 binding single-stranded DNA binding ATP-dependent DNA helicase activity helicase activity protein binding ATP binding ATP-dependent helicase activity four-way junction helicase activity ATPase activity annealing helicase activity ATP-dependent 3'-5' DNA helicase activity G-quadruplex DNA binding
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity double-strand break repair via homologous recombination DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement DNA strand renaturation DNA replication DNA repair DNA recombination cellular response to DNA damage stimulus mitotic G2 DNA damage checkpoint response to X-ray protein sumoylation replication fork processing DNA duplex unwinding positive regulation of transcription, DNA-templated negative regulation of DNA recombination replication fork protection protein oligomerization negative regulation of cell division cellular response to ionizing radiation cellular response to hydroxyurea cellular response to camptothecin regulation of signal transduction by p53 class mediator
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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