Invitrogen

Biotin Recombinant Mouse Monoclonal Antibody (muM33)

Name: Biotin Recombinant Mouse Monoclonal Antibody (muM33)
Brand: Invitrogen
SKU: MA5-51923

View all (86) Biotin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

Biotin Antibody (MA5-51923) in ELISA

ELISA analysis of Biotin using Biotin Recombinant Mouse Monoclocal Antibody (Product # MA5-51923; red line) and isotype control (Product # MA5-47752, anti-fluorescein; orange line). ELISA plate was coated with biotin at a concentration of 2.5 µg/mL and a 3-fold serial dilution from 3000 to 0.0169 ng/mL was performed using primary antibody. For signal detection, a 1:4,000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.

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Product Details

MA5-51923

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

muM33

Immunogen

The original antibody was generated by immunizing BALB/cmice with biotin tagged protien.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_3250615

Product Specific Information

This antibody recognizes and binds biotin.

Target Information

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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