Invitrogen

BMPR2 Monoclonal Antibody (3F6F8)

Name: BMPR2 Monoclonal Antibody (3F6F8)
Brand: Invitrogen
SKU: MA5-15827

Advanced Verification

8 Published Figures

10 References

View all (17) BMPR2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

BMPR2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

BMPR2 Antibody (MA5-15827) in ICC/IF

Immunofluorescent analysis of BMPR2 (green) in HepG2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20c, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a BMPR2 mouse monoclonal antibody (Product # MA5-15827) at a concentration of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32723) at a dilution of 1... View More

BMPR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BMPR2 Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

BMPR2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

BMPR2 Antibody — Fig. 3 Antibody 3F6 has no effect on BMP-responsiveness in BMPR2 knock-down HEK293T cells. a Expression levels of endogenous BMPR2 in scramble control HEK293T cells (Control) and HEK293T cells carrying anti-BMPR2 shRNA (BMPR2-KD) compared to HPRT1 loading control. Data are expressed as normalized to scramble control (Control) using the 2^-ΔΔCt method. n= 6 per condition. Asterisk indicates p< 0.05 by unpaired t test. b, c Expression of endogenous BMPR2 in scramble control HEK293T cells (Control) and BMPR2-KD HEK293T cells compared to β-actin loading control. Approximate molecular weights are indicated in b. Representative immunoblot is shown in b and results from three independent runs are quantified in c (data are expressed as mean ± SEM ratio of BMPR2: β-actin normalized to scramble control (Relative Expression)). Asterisk indicates p< 0.05 by paired t test. d, e BMP2 induces phosphorylation of SMAD1, 5, and 8 (pSMAD1,5,8) in BMPR2-KD HEK293T cells. No effect on BMP2-responsiveness of BMPR2-KD HEK293T cells was observed with pre-treatment using control ascites (Ctrl Asc.) or 3F6. Approximate molecular weights are indicated in d. Results are quantified in E and expressed as mean ± SEM ratio of phosphorylated SMAD1,5,8: total SMAD1 relative to BMP2 treatment alone (Relative pS1:S1). n= 4 per condition {KD}

Product Details

MA5-15827

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 ug/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:200

Flow Cytometry (Flow)

1:100-1:200

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3F6F8

Immunogen

Purified recombinant fragment of human BMPR2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11156844

Product Specific Information

MA5-15827 targets BMPR2 in FACS, ICC/IF, IHC (P), and WB applications and shows reactivity with Human, mouse, Non-human primate, and rat samples.

Target Information

BMPR2 is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs that are involved in endochondral bone formation and embryogenesis. The loss of interaction and lack of phosphorylation of TCTEL1 by BMPR2 may contribute to the pathogenesis of primary pulmonary hypertension (PPH) and BMPR2 also plays an essential role in human T-cell differentiation. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in BMPR2 have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BMP type II receptor; BMP type-2 receptor; BMPR-2; Bone morphogenetic protein receptor type II; Bone morphogenetic protein receptor type-2; bone morphogenetic protein receptor, type II (serine/threonine kinase); bone morphogenic protein receptor, type II (serine/threonine kinase); BRK-3; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4

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Gene Aliases: 2610024H22Rik; AL117858; AW546137; BB189135; BMP-2; BMPR-2; BMPR-II; BMPR2; BMPR3; BMPRII; BMR2; BRK-3; Gm20272; POVD1; PPH1; T-ALK

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UniProt ID: (Human) Q13873, (Mouse) O35607

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Entrez Gene ID: (Human) 659, (Rat) 140590, (Mouse) 12168

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Function(s)

receptor signaling protein serine/threonine kinase activity protein binding ATP binding activin receptor activity, type II growth factor binding BMP binding metal ion binding BMP receptor activity transmembrane receptor protein serine/threonine kinase activity transforming growth factor beta-activated receptor activity nucleotide binding protein kinase activity protein serine/threonine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BMPR2 Monoclonal Antibody (3F6F8)

BMPR2 Antibody (MA5-15827) in ICC/IF

Product Details

MA5-15827

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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