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  • BAMBI Antibodies

Invitrogen

BAMBI Polyclonal Antibody

Advanced Verification
View all (18) BAMBI antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite BAMBI Polyclonal Antibody

  • Antibody Testing Data (2)
  • Advanced Verification (1)
BAMBI Antibody in Western Blot (WB)
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BAMBI Antibody in Western Blot (WB)
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BAMBI Antibody (PA5-80372) in WB

Western blot was performed using Anti-BAMBI Polyclonal Antibody (Product # PA5-80372) and a 40 kDa band corresponding to BMP and activin membrane-bound inhibitor homolog was observed across tissues tested. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Brain (Lane 2), Mouse Skeletal Muscle (Lane 3), Mouse Mammary gland (Lane 4) and Rat Mammary gland (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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BAMBI Antibody in Western Blot (WB)
BAMBI Antibody in Western Blot (WB)
BAMBI Antibody
BAMBI Polyclonal Antibody

Product Details

PA5-80372

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1:25,000-1:50,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human BAMBI protein (Met1-Ala152)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787696

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human BAMBI/NMA.

Target Information

BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development. BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BMP and activin membrane-bound inhibitor homolog; BMP and activin membrane-bound inhibitor, homolog; Kinase-deficient TGFbeta superfamily receptor subunit; Non-metastatic gene A protein; Putative transmembrane protein NMA

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Gene Aliases: 2610003H06Rik; BAMBI; NMA

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UniProt ID: (Human) Q13145, (Rat) Q91XN4, (Mouse) Q9D0L6

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Entrez Gene ID: (Human) 25805, (Rat) 83837, (Mouse) 68010

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Function(s)
frizzled binding type II transforming growth factor beta receptor binding
Process(es)
transforming growth factor beta receptor signaling pathway positive regulation of cell proliferation regulation of cell shape positive regulation of epithelial to mesenchymal transition cell migration negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of protein binding positive regulation of catenin import into nucleus positive regulation of transcription, DNA-templated positive regulation of canonical Wnt signaling pathway
It has to be done as per old AB suggested Products section.
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