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  • BAMBI Antibodies

Invitrogen

BAMBI Polyclonal Antibody

View all (18) BAMBI antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite BAMBI Polyclonal Antibody

  • Antibody Testing Data (1)
BAMBI Antibody in Western Blot (WB)
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BAMBI Antibody in Western Blot (WB)
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BAMBI Antibody (PA5-109443) in WB

Western blot analysis of BAMBI in extracts of various cell lines. Samples were incubated with BAMBI Polyclonal antibody (Product # PA5-109443) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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BAMBI Antibody in Western Blot (WB)
BAMBI Polyclonal Antibody

Product Details

PA5-109443

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-152 of human BAMBI (NP_036474.1).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2854854

Product Specific Information

Immunogen sequence: VLLTKGEIRC YCDAAHCVAT GYMCKSELSA CFSRLLDPQN SNSPLTHGCL DSLASTTDIC QAKQARNHSG TTIPTLECCH EDMCNYRGLH DVLSPPRGEA SGQGNRYQHD GSRNLITKVQ ELTSSKELWF RA

Target Information

BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development. BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BMP and activin membrane-bound inhibitor homolog; BMP and activin membrane-bound inhibitor, homolog; Kinase-deficient TGFbeta superfamily receptor subunit; Non-metastatic gene A protein; Putative transmembrane protein NMA

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Gene Aliases: 2610003H06Rik; BAMBI; NMA

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UniProt ID: (Human) Q13145, (Rat) Q91XN4, (Mouse) Q9D0L6

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Entrez Gene ID: (Human) 25805, (Rat) 83837, (Mouse) 68010

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Function(s)
frizzled binding type II transforming growth factor beta receptor binding
Process(es)
transforming growth factor beta receptor signaling pathway positive regulation of cell proliferation regulation of cell shape positive regulation of epithelial to mesenchymal transition cell migration negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of protein binding positive regulation of catenin import into nucleus positive regulation of transcription, DNA-templated positive regulation of canonical Wnt signaling pathway
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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