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  • Aurora A Antibodies

Invitrogen

Aurora A Polyclonal Antibody

View all (41) Aurora A antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Aurora A Polyclonal Antibody

  • Antibody Testing Data (1)
Aurora A Antibody in Western Blot (WB)
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Aurora A Antibody in Western Blot (WB)
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Aurora A Antibody (PA5-95055) in WB

Western blot analysis of Aurora A in mouse ovary tissue lysate (50 µg). Samples were incubated with Aurora A polyclonal antibody (Product # PA5-95055) using a 0.5 µg/mL dilution. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Aurora A Antibody in Western Blot (WB)
Aurora A Polyclonal Antibody

Product Details

PA5-95055

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse Aurora A (109-125aa QKTEDTKKRQWTLEDFD).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806861

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: Mouse Ovary Tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis by association in vivo with centrosomes. Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ARK-1; AURAK; Aurora 2; aurora A; Aurora A Kinase; aurora family kinase 1; Aurora kinase A; aurora-A; aurora-related kinase 1; aurora/IP; Aurora/IPL1-related kinase 1; AURORA2; EC 2.7.11.1; hARK1; Ipl1- and aurora-related kinase 1; IPL1-related kinase; MGC34538; OTTHUMP00000031341; OTTHUMP00000166072; ratAurA; RP5-1167H4.6; serine/threonine kinase 6; serine/threonine protein kinase 6; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase Ayk1

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Gene Aliases: Aik; AIRK1; ARK-1; Ark1; AU019385; Aura; Aurka; Aurora-A; AW539821; Ayk1; Btak; IAK; IAK1; Stk15; Stk6

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UniProt ID: (Mouse) P97477

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Entrez Gene ID: (Mouse) 20878, (Rat) 261730

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding ubiquitin protein ligase binding histone serine kinase activity
Process(es)
meiotic spindle organization microtubule cytoskeleton organization mitotic cell cycle protein phosphorylation cell cycle spindle organization mitotic spindle organization spindle assembly involved in female meiosis I mitotic nuclear division mitotic centrosome separation meiotic nuclear division anterior/posterior axis specification phosphorylation cell projection organization regulation of protein stability negative regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of cytokinesis histone-serine phosphorylation negative regulation of apoptotic process centrosome organization cell division centrosome localization protein localization to centrosome negative regulation of spindle checkpoint positive regulation of oocyte maturation neuron projection extension response to estradiol
It has to be done as per old AB suggested Products section.
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