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  • Angiotensinogen Antibodies

Invitrogen

Angiotensinogen Polyclonal Antibody

Advanced Verification
1 Published Figure
1 Reference
View all (42) Angiotensinogen antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Angiotensinogen Polyclonal Antibody

  • Antibody Testing Data (8)
  • Published Figures (1)
  • Advanced Verification (1)
Angiotensinogen Antibody in Immunocytochemistry (ICC/IF)
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Angiotensinogen Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 10

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Angiotensinogen Antibody (PA5-21520) in ICC/IF

Immunofluorescence analysis of Angiotensinogen was performed using 70% confluent log phase Hep G2 cells treated with 1X protein transport inhibitor (Product # 00-4980-03) for 4 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. cells were labeled with Angiotensinogen Polyclonal Ant... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Angiotensinogen Antibody in Immunocytochemistry (ICC/IF)
Angiotensinogen Antibody in Immunocytochemistry (ICC/IF)
Angiotensinogen Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Angiotensinogen Antibody in Western Blot (WB)
Angiotensinogen Antibody in Western Blot (WB)
Angiotensinogen Antibody in Western Blot (WB)
Angiotensinogen Antibody in Western Blot (WB)
Angiotensinogen Antibody in Western Blot (WB)
Angiotensinogen Antibody in Immunocytochemistry (ICC/IF)
Angiotensinogen Antibody

Product Details

PA5-21520

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Angiotensinogen. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153449

Product Specific Information

Recommended positive controls: HepG2, human plasma, mouse plasma, rat plasma.

Predicted reactivity: Chimpanzee (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by AGT, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha-1 antiproteinase, antitrypsin; angiotensin I; angiotensin II; angiotensin ll; Angiotensinogen; angiotensinogen (PAT); angiotensinogen (serpin peptidase inhibitor, clade A, member 8); pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; serpin peptidase inhibitor, clade A, member 8

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Gene Aliases: AGT; AI265500; Ang; AngI; AngII; ANHU; ANRT; Aogen; PAT; SERPINA8

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UniProt ID: (Human) P01019, (Rat) P01015

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Entrez Gene ID: (Human) 183, (Rat) 24179, (Mouse) 11606

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Function(s)
serine-type endopeptidase inhibitor activity hormone activity protein binding growth factor activity type 1 angiotensin receptor binding type 2 angiotensin receptor binding sodium channel regulator activity angiotensin receptor binding
Process(es)
regulation of cell growth positive regulation of cytokine production kidney development blood vessel remodeling angiotensin maturation regulation of blood volume by renin-angiotensin renin-angiotensin regulation of aldosterone production regulation of renal output by angiotensin regulation of heart rate regulation of blood vessel size by renin-angiotensin renal system process angiotensin-mediated drinking behavior positive regulation of extracellular matrix constituent secretion cellular sodium ion homeostasis cell surface receptor signaling pathway G-protein coupled receptor signaling pathway G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger phospholipase C-activating G-protein coupled receptor signaling pathway activation of phospholipase C activity nitric oxide mediated signal transduction cell-cell signaling female pregnancy aging regulation of blood pressure positive regulation of activation of JAK2 kinase activity positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy positive regulation of macrophage derived foam cell differentiation positive regulation of cholesterol esterification negative regulation of endopeptidase activity regulation of norepinephrine secretion positive regulation of phosphatidylinositol 3-kinase signaling artery smooth muscle contraction response to muscle activity involved in regulation of muscle adaptation negative regulation of angiogenesis regulation of vasoconstriction negative regulation of cell growth positive regulation of cellular protein metabolic process positive regulation of superoxide anion generation positive regulation of NAD(P)H oxidase activity negative regulation of tissue remodeling low-density lipoprotein particle remodeling catenin import into nucleus regulation of renal sodium excretion positive regulation of renal sodium excretion regulation of cell proliferation vasodilation positive regulation of nitric oxide biosynthetic process positive regulation of epidermal growth factor receptor signaling pathway positive regulation of blood pressure positive regulation of transcription, DNA-templated positive regulation of vasodilation fibroblast proliferation positive regulation of fibroblast proliferation regulation of long-term neuronal synaptic plasticity smooth muscle cell proliferation cytokine secretion positive regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation positive regulation of NF-kappaB transcription factor activity negative regulation of neurotrophin TRK receptor signaling pathway stress-activated MAPK cascade regulation of calcium ion transport regulation of transmission of nerve impulse cell growth involved in cardiac muscle cell development positive regulation of protein tyrosine kinase activity ERK1 and ERK2 cascade uterine smooth muscle contraction cellular response to mechanical stimulus positive regulation of branching involved in ureteric bud morphogenesis regulation of extracellular matrix assembly positive regulation of gap junction assembly regulation of cardiac conduction positive regulation of reactive oxygen species metabolic process positive regulation of extrinsic apoptotic signaling pathway ovarian follicle rupture blood vessel development branching involved in ureteric bud morphogenesis regulation of systemic arterial blood pressure by circulatory renin-angiotensin angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure brain renin-angiotensin system cell-matrix adhesion activation of NF-kappaB-inducing kinase activity excretion establishment of blood-nerve barrier positive regulation of cell proliferation negative regulation of cell proliferation response to cold response to salt stress regulation of gene expression positive regulation of gene expression extracellular matrix organization peristalsis positive regulation of peptidyl-serine phosphorylation positive regulation of multicellular organism growth vasoconstriction hormone metabolic process drinking behavior regulation of apoptotic process positive regulation of catalytic activity positive regulation of MAPK cascade negative regulation of neuron apoptotic process positive regulation of fatty acid biosynthetic process positive regulation of organ growth astrocyte activation regulation of inflammatory response smooth muscle cell differentiation positive regulation of protein kinase C activity positive regulation of membrane hyperpolarization
It has to be done as per old AB suggested Products section.
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