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  • Alpaca anti-Human/Rabbit IgG Fc Secondary Antibodies

Chromotek

Alpaca anti-Human IgG/rabbit IgG, Fc-specific VHH, Nano-CaptureLigand™ Recombinant Secondary Antibody, Biotin

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Cite Alpaca anti-Human IgG/rabbit IgG, Fc-specific VHH, Nano-CaptureLigand™ Recombinant Secondary Antibody, Biotin

  • Antibody Testing Data (6)
Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody in ELISA (ELISA)
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Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody in ELISA (ELISA)
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Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody (SHURBGB-1-100) in ELISA

Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (Product # SHURBGB-1-100) (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and seco... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody in ELISA (ELISA)
Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody in ELISA (ELISA)
Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody in ELISA (ELISA)
Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody in Functional Assay (Functional)
Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody in Functional Assay (Functional)
Human IgG/rabbit IgG, Fc-specific VHH Secondary Antibody in Functional Assay (Functional)

Product Details

SHURBGB-1-100

Applications
Tested Dilution

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent
Product Specifications

Species Reactivity

Human, Rabbit

Host/Isotype

Alpaca

Expression System

E. coli

Class

Recombinant Monoclonal

Type

Secondary Antibody

Clone

CTK0101

Immunogen

rabbit IgG

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

25mM TAPS, pH 8.5, with 5mM EDTA, 500mM NaCl

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Wet ice

Target

IgG Fc

Antibody Form

VHH

Product Specific Information

Anti-human IgG and rabbit IgG Nanobody for site-directed immobilization of human IgG and rabbit IgG on plates, surfaces and biosensors.

Affinity: Dissociation constant KD of Human IgG1: 1.4 nM; Human IgG2: 0.4 nM; Human IgG3: 9.0 nM; Human IgG4: 0.2 nM. Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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