Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Secondary Antibodies ›
  • Alpaca anti-Human/Rabbit IgG Secondary Antibodies

Chromotek

Alpaca anti-Human IgG/Rabbit IgG VHH, Nano-Secondaries™ Recombinant Secondary Antibody, Alexa Fluor™ 488

View all (4) Alpaca anti-Human/Rabbit IgG antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Create Citation
Questions & Answers
Datasheet
Create Citation
Questions & Answers

Cite Alpaca anti-Human IgG/Rabbit IgG VHH, Nano-Secondaries™ Recombinant Secondary Antibody, Alexa Fluor™ 488

  • Antibody Testing Data (4)
Human IgG/Rabbit IgG VHH Secondary Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.
Human IgG/Rabbit IgG VHH Secondary Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.

FIGURE: 1 / 4

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

Human IgG/Rabbit IgG VHH Secondary Antibody (SRBAF488-1-100) in ICC/IF

HeLa cells were immunostained with rabbit anti-Lamin B1 antibodies and alpaca anti-rabbit IgG VHH Alexa Fluor® 568 (1:1,000). Confocal and gated STED images were acquired with a Leica TCS SP8 STED 3X microscope, pulsed depletion with a 775 nm laser. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

Human IgG/Rabbit IgG VHH Secondary Antibody in Immunocytochemistry (ICC/IF)
Human IgG/Rabbit IgG VHH Secondary Antibody in Immunocytochemistry (ICC/IF)
Human IgG/Rabbit IgG VHH Secondary Antibody in Western Blot (WB)
Human IgG/Rabbit IgG VHH Secondary Antibody in Western Blot (WB)

Product Details

SRBAF488-1-100

Applications
Tested Dilution

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:1,000
Product Specifications

Species Reactivity

Human, Rabbit

Host/Isotype

Alpaca

Expression System

E. coli

Class

Recombinant Monoclonal

Type

Secondary Antibody

Clone

CTK0101, CTK0102

Immunogen

rabbit IgG

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
  • Alexa Fluor 568 
  • Alexa Fluor 647 
  • Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

10mM HEPES, pH 7, with 5mM EDTA, 500mM NaCl

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Target

IgG

Antibody Form

VHH

Product Specific Information

Anti-human IgG and rabbit IgG Nanobody for detection of human IgG and rabbit IgG in Immunofluorescence and Western Blot.

Affinity: CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody SRBAF488-1-100 on CiteAb

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-b648c57dc-dsdl2:80/100.66.129.203:80.
git-commit: 561b23eae95443db19074e56c849bd303926a883
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.1-2025.09.37-1.0