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  • Alix Antibodies

Invitrogen

Alix Polyclonal Antibody

Advanced Verification
3 References
View all (23) Alix antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Alix Polyclonal Antibody

  • Antibody Testing Data (8)
  • Advanced Verification (2)
Alix Antibody in Immunocytochemistry (ICC/IF)
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Alix Antibody in Immunocytochemistry (ICC/IF)
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Alix Antibody (PA5-52873) in ICC/IF

Immunofluorecent analysis of Alix in human cell line U-2 OS using Alix Polyclonal Antibody (Product # PA5-52873). Staining shows localization to cytosol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Alix Antibody in Immunocytochemistry (ICC/IF)
Alix Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Alix Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Alix Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Alix Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Alix Antibody in Western Blot (WB)
Alix Antibody in Western Blot (WB)
Alix Antibody in Western Blot (WB)
Alix Antibody
Alix Antibody
Alix Polyclonal Antibody

Product Details

PA5-52873

Applications
Tested Dilution
Publications

Western Blot (WB)

0.04-0.4 µg/mL
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000
-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL
-

Affinity Purification (AP)

-
- -
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Nematode

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Alix. Recombinant protein control fragment (Product #RP-90648).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637865

Product Specific Information

Immunogen sequence: VPVSVQQSLA AYNQRKADLV NRSIAQMREA TTLANGVLAS LNLPAAIEDV SGDTVPQSIL TKSRSVIEQG GIQTVDQLIK ELPELLQRNR EILDESLRLL DEEEATDND

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.

Target Information

Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ALG-2 interacting protein 1; ALG-2-interacting protein 1; ALG-2-interacting protein X; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; Hp95; MGC17003; PDCD6-interacting protein; Programmed cell death 6-interacting protein

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Gene Aliases: AIP1; ALIX; DRIP4; HP95; KIAA1375; PDCD6IP

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UniProt ID: (Human) Q8WUM4

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Entrez Gene ID: (Human) 10015

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Function(s)
protein binding proteinase activated receptor binding protein homodimerization activity calcium-dependent protein binding
Process(es)
cell separation after cytokinesis apoptotic process nucleus organization mitotic metaphase plate congression regulation of centrosome duplication protein transport viral life cycle multivesicular body assembly viral budding via host ESCRT complex ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway regulation of mitotic spindle assembly positive regulation of exosomal secretion regulation of extracellular exosome assembly positive regulation of extracellular exosome assembly
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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