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  • Alexa Fluor 488 Antibodies
Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Invitrogen

Alexa Fluor 488 Recombinant Superclonal™ Antibody

Advanced Verification
8 References
View all (3) Alexa Fluor 488 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Alexa Fluor 488 Recombinant Superclonal™ Antibody

  • Antibody Testing Data (3)
  • Advanced Verification (1)
Alexa Fluor 488 Antibody in Flow Cytometry (Flow)
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Alexa Fluor 488 Antibody in Flow Cytometry (Flow)
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Alexa Fluor 488 Antibody (710369) in Flow

Fixed and permeabilized HeLa cells were stained with MMP16/sRAGE Recombinant Rabbit Superclonal™ Antibody (Product # 710369), followed by Alexa Fluor 488 Goat anti-Rabbit Ig (right peak). Quenching was performed on these labelled cells using Alexa Fluor 488 Recombinant Rabbit Superclonal™ Antibody (middle peak). Isotype control (left peak). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Alexa Fluor 488 Antibody in Flow Cytometry (Flow)
Alexa Fluor 488 Antibody in Flow Cytometry (Flow)
Alexa Fluor 488 Antibody in Fluorescent Quenching (FQ)
Alexa Fluor 488 Antibody

Product Details

710369

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent
View 4 publications 4 publications

Fluorescent Quenching (FQ)

Assay-Dependent
View 1 publication 1 publication
Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Immunogen

Alexa Fluor® 488 dye conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532697

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes. The Alexa Fluor® 488 dye possesses near identical spectral properties and quantum yield to fluorescein isothiocyanate (FITC), but produces brighter, more photostable conjugates. These conjugates are used for imaging and other applications requiring increased sensitivity without being overly impacted by environmental factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

It has to be done as per old AB suggested Products section.
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