Invitrogen

Adiponectin Recombinant Rabbit Monoclonal Antibody (11H4L4)

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Datasheet
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Datasheet
Protocols
Questions & Answers
Adiponectin Recombinant Rabbit Monoclonal Antibody (11H4L4)

Product Details

701148

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20
-

Immunocytochemistry (ICC/IF)

1-3 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

11H4L4

Immunogen

Recombinant protein corresponding to amino acids 57-244 of human adiponectin
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532410

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 30 kDa adipocyte complement-related protein; ACRP30; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; Adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; Adiponectin; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADPIOQ; apM-1; Gelatin-binding protein; gelatin-binding protein 28

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Gene Aliases: 30kDa; ACDC; ACRP30; Ad; adipo; ADIPOQ; ADIPQTL1; ADPN; APM-1; APM1; APN; GBP28

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UniProt ID: (Human) Q15848, (Mouse) Q60994

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Entrez Gene ID: (Human) 9370, (Rat) 246253, (Mouse) 11450

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Function(s)
receptor binding cytokine activity hormone activity protein binding sialic acid binding identical protein binding protein homodimerization activity
Process(es)
response to hypoxia positive regulation of protein phosphorylation glucose metabolic process fatty acid beta-oxidation response to nutrient circadian rhythm response to sucrose response to glucose positive regulation of signal transduction negative regulation of platelet-derived growth factor receptor signaling pathway positive regulation of protein kinase A signaling negative regulation of macrophage derived foam cell differentiation negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol efflux regulation of glucose metabolic process response to activity negative regulation of smooth muscle cell migration fatty acid oxidation negative regulation of cell migration negative regulation of granulocyte differentiation response to nutrient levels negative regulation of protein autophosphorylation positive regulation of cellular protein metabolic process negative regulation of tumor necrosis factor production positive regulation of interleukin-8 production cellular response to insulin stimulus positive regulation of myeloid cell apoptotic process adiponectin-activated signaling pathway negative regulation of heterotypic cell-cell adhesion low-density lipoprotein particle clearance response to tumor necrosis factor cellular response to drug response to drug glucose homeostasis positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAP kinase activity response to ethanol negative regulation of fat cell differentiation negative regulation of macrophage differentiation negative regulation of low-density lipoprotein particle receptor biosynthetic process negative regulation of gluconeogenesis negative regulation of blood pressure positive regulation of blood pressure positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of fatty acid metabolic process positive regulation of glucose import negative regulation of hormone secretion negative regulation of smooth muscle cell proliferation negative regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation negative regulation of phagocytosis negative regulation of synaptic transmission brown fat cell differentiation protein homooligomerization response to glucocorticoid membrane depolarization membrane hyperpolarization protein heterotrimerization negative regulation of ERK1 and ERK2 cascade response to linoleic acid detection of oxidative stress cellular response to cAMP positive regulation of monocyte chemotactic protein-1 production cellular response to epinephrine stimulus protein localization to plasma membrane negative regulation of intracellular protein transport negative regulation of receptor binding negative regulation of DNA biosynthetic process positive regulation of glycogen (starch) synthase activity positive regulation of metanephric glomerular visceral epithelial cell development positive regulation of cAMP-dependent protein kinase activity positive regulation of renal albumin absorption negative regulation of platelet-derived growth factor receptor-alpha signaling pathway negative regulation of metanephric mesenchymal cell migration generation of precursor metabolites and energy
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