Invitrogen

Actin Monoclonal Antibody (A2E1)

View all (21) Actin antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers
Actin Monoclonal Antibody (A2E1)

Product Details

MA5-42536

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:10000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A2E1

Immunogen

Synthetic peptide corresponding to N terminal of Human Alpha actin (smooth muscle, skeletal muscle, cardiac muscle)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911677

Product Specific Information

Positive Control: Rat heart tissue lysate, mouse smooth muscle tissue lysate, human skeletal muscle tissue lysate.

Subcellular Location: Cytoplasm, Cytoskeleton

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; beta-actin; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E430023M04Rik; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; VSCM

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UniProt ID: (Human) P68133, (Human) P62736, (Human) P60709, (Human) P68032, (Human) P63261, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710, (Mouse) P68033, (Rat) P68035, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269

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Entrez Gene ID: (Human) 58, (Human) 59, (Human) 60, (Human) 70, (Human) 71, (Human) 72, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Mouse) 11464, (Rat) 29275, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365

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Function(s)
structural constituent of cytoskeleton protein binding ATP binding myosin binding ADP binding protein kinase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding ATPase activity ubiquitin protein ligase binding nucleotide binding molecular_function
Process(es)
apoptotic process positive regulation of gene expression actin filament-based movement muscle filament sliding skeletal muscle thin filament assembly actomyosin structure organization actin-myosin filament sliding response to drug negative regulation of apoptotic process response to ethanol cardiac myofibril assembly cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction mesenchyme migration retina homeostasis movement of cell or subcellular component cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis sarcomere organization vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation muscle contraction regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development skeletal muscle fiber development axonogenesis substantia nigra development ATP-dependent chromatin remodeling retina development in camera-type eye cellular response to electrical stimulus metabolic process actin-mediated cell contraction response to mechanical stimulus response to extracellular stimulus response to lithium ion cell growth skeletal muscle fiber adaptation response to steroid hormone biological_process cytoskeleton organization positive regulation of gene expression, epigenetic response to calcium ion
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