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  • Primary Antibodies ›
  • H3K18ac Antibodies
Antibody was validated by peptide array and recommended for denaturing applications. SNAP-ChIP™ spike-in validated H3K18ac antibody is recommended for ChIP.

Invitrogen

H3K18ac Polyclonal Antibody

Advanced Verification
View all (17) H3K18ac antibodies

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Datasheet
Protocols
Questions & Answers

Cite H3K18ac Polyclonal Antibody

  • Antibody Testing Data (3)
  • Advanced Verification (1)
H3K18ac Antibody in Immunocytochemistry (ICC/IF)
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H3K18ac Antibody in Immunocytochemistry (ICC/IF)
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H3K18ac Antibody (720095) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized HeLa cells for detection of Histone H3K18ac using Anti-Histone H3K18ac Rabbit Polyclonal Antibody (Product # 720095, 0.5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:400). Panel a) shows representative cells that were stained for detection and localization of Histone H3K18ac protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product #... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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H3K18ac Antibody in Immunocytochemistry (ICC/IF)
H3K18ac Antibody in Western Blot (WB)
H3K18ac Antibody in Peptide array (ARRAY)
H3K18ac Antibody
H3K18ac Polyclonal Antibody

Product Details

720095

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

0.5 µg/mL
-

Peptide Array (Array)

0.25 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Acetylated peptide (Lys18) corresponding to Human H3 (aa 15-22)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% BSA, 30% glycerol

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532804

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

Since it is conserved across species, the antibody may react with many other species.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acetyl H3; Acetyl Histone H3; Acetyl-H3 K18; Acetyl-H3 Lys18; Acetyl-Histone H3 K18; Acetyl-Histone H3 Lys18; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K18ac; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3/b; H3/d; H3/f; H3/j; H3K18; H3Lys18ac; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; Histone 3; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K18

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Gene Aliases: H3; H3.2; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; HIST1H3A; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D

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UniProt ID: (Human) P68431, (Human) Q71DI3

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961

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Function(s)
DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion
Process(es)
chromatin silencing at rDNA nucleosome assembly blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic positive regulation of defense response to virus by host DNA replication-dependent nucleosome assembly telomere organization protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy
It has to be done as per old AB suggested Products section.
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