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  • ATP Synthase beta Antibodies

Invitrogen

ATP Synthase beta Monoclonal Antibody (4.3E8.D10)

4 Published Figures
11 References
View all (43) ATP Synthase beta antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ATP Synthase beta Monoclonal Antibody (4.3E8.D10)

  • Antibody Testing Data (7)
  • Published Figures (4)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
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ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
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ATP Synthase beta Antibody (MA1-930) in ICC/IF

Immunofluorescent analysis of ATP Synthase beta (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without ATP Synthase beta mouse monoclonal antibody (Product # MA1-930), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Western Blot (WB)
ATP Synthase beta Antibody in Immunoprecipitation (IP)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)
ATP Synthase beta Antibody in Immunohistochemistry (IHC)
ATP Synthase beta Antibody in Immunohistochemistry (IHC)
ATP Synthase beta Monoclonal Antibody (4.3E8.D10)

Product Details

MA1-930

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 4 publications 4 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 6 publications 6 publications

Immunoprecipitation (IP)

2-5 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4.3E8.D10

Immunogen

Intact rat mitochondria.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated
  • Alexa Fluor 488 
  • Alexa Fluor 555 
  • Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2227740

Product Specific Information

MA1-930 detects the beta subunit of ATP synthase from mouse rat and human samples. This antibody is useful as a mitochondrial marker.

MA1-930 has been successfully used in immunofluorescence, western blot, and immunoprecipitation procedures. By immunoprecipitation, this antibody detects an 50 kDa protein representing ATP synthase from solubilized rat brain mitochondria.

Target Information

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP synthase F(1) complex catalytic subunit beta, mitochondrial; ATP synthase F(1) complex subunit beta, mitochondrial; ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; epididymis secretory protein Li 271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit

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Gene Aliases: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271

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UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480

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Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947

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Function(s)
transporter activity protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism calcium ion binding lipoprotein particle receptor activity nucleotide binding hydrolase activity hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
Process(es)
angiogenesis osteoblast differentiation generation of precursor metabolites and energy lipid metabolic process ATP biosynthetic process negative regulation of cell adhesion involved in substrate-bound cell migration mitochondrion organization ATP hydrolysis coupled proton transport proton transport mitochondrial ATP synthesis coupled proton transport regulation of intracellular pH receptor-mediated endocytosis ATP synthesis coupled proton transport ATP metabolic process transport ion transport hydrogen ion transmembrane transport
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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