Bioss

APAF1CT Polyclonal Antibody

Name: APAF1CT Polyclonal Antibody
Brand: Bioss
SKU: BS-0058R

View all (20) APAF1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

APAF1CT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 1

APAF1CT Antibody (BS-0058R) in IHC (P)

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti APAF1 Polyclonal Antibody, Unconjugated (bs-0058R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

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Immunogen image

Immunogen Image APAF1CT Polyclonal Antibody

Product Details

BS-0058R

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Apaf-1, amino acids 1201-1248.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APAF-1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; DKFZp781B1145; forebrain overgrowth

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Gene Aliases: 6230400I06Rik; APAF-1; APAF1; Apaf1l; CED4; fog; KIAA0413; mKIAA0413; zgc:136543

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UniProt ID: (Human) O14727, (Rat) Q9EPV5, (Mouse) O88879, (Zebrafish) Q9I9H8

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Entrez Gene ID: (Human) 317, (Rat) 78963, (Mouse) 11783, (Zebrafish) 58131

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Function(s)

nucleotide binding protein binding ATP binding cysteine-type endopeptidase activator activity involved in apoptotic process heat shock protein binding identical protein binding ADP binding peptidase activity hydrolase activity

Process(es)

proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c regulation of apoptotic process apoptotic signaling pathway response to hypoxia neural tube closure activation of cysteine-type endopeptidase activity involved in apoptotic process multicellular organism development brain development aging response to nutrient cardiac muscle cell apoptotic process cell differentiation forebrain development glial cell apoptotic process protein homooligomerization neuron apoptotic process cellular response to transforming growth factor beta stimulus regulation of apoptotic DNA fragmentation positive regulation of apoptotic signaling pathway nervous system development positive regulation of apoptotic process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress response to G1 DNA damage checkpoint signaling intrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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APAF1CT Polyclonal Antibody

APAF1CT Antibody (BS-0058R) in IHC (P)

Product Details

BS-0058R

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

References (0)

Bioinformatics

Performance Guarantee

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