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  • Primary Antibodies ›
  • AP2A1 Antibodies

Invitrogen

AP2A1 Polyclonal Antibody

View all (8) AP2A1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite AP2A1 Polyclonal Antibody

  • Antibody Testing Data (1)
AP2A1 Antibody in Western Blot (WB)
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AP2A1 Antibody in Western Blot (WB)
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AP2A1 Antibody (PA5-109397) in WB

Western blot analysis of AP2A1 in extracts of various cell lines. Samples were incubated with AP2A1 Polyclonal antibody (Product # PA5-109397) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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AP2A1 Antibody in Western Blot (WB)
AP2A1 Polyclonal Antibody

Product Details

PA5-109397

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human AP2A1 (NP_055018.2).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.24 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2854808

Product Specific Information

Immunogen sequence: MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV LVNSNSELIR LINNAIKNDL ASRNPTFMCL ALHCIANVGS REMGEAFAAD IPRILVAGDS MDSVKQSAAL CLLRLYKASP DLVPMGEWTA RVVHLLNDQH MGVVTAAVSL ITCLCKKNPD DFKTCVSLAV SRLSRIVSSA STDLQDYTYY FVPAPWLSVK

Target Information

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; Adaptor protein complex AP-2 subunit alpha-1; adaptor protein complex AP-2, alpha 1 subunit; adaptor related protein complex 2, alpha 1 subunit; Adaptor-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; adaptor-related protein complex AP-2, alpha 1 subunit; Alpha-adaptin A; Alpha1-adaptin; AP-2 complex subunit alpha-1; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

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Gene Aliases: ADTAA; AP2-ALPHA; AP2A1; CLAPA1

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UniProt ID: (Human) O95782, (Mouse) P17426

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Entrez Gene ID: (Human) 160, (Rat) 308578, (Mouse) 11771

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Function(s)
protein binding protein C-terminus binding protein transporter activity protein kinase binding protein complex binding clathrin adaptor activity
Process(es)
intracellular protein transport biological_process vesicle-mediated transport negative regulation of hyaluronan biosynthetic process Golgi to endosome transport endocytosis microtubule-based movement antigen processing and presentation of exogenous peptide antigen via MHC class II negative regulation of epidermal growth factor receptor signaling pathway ephrin receptor signaling pathway regulation of defense response to virus by virus Wnt signaling pathway, planar cell polarity pathway clathrin-mediated endocytosis transport protein transport
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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