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  • ALOX15 Antibodies

OriGene

ALOX15 Monoclonal Antibody (OTI8C5), TrueMAB™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ALOX15 Monoclonal Antibody (OTI8C5), TrueMAB™

  • Antibody Testing Data (1)
ALOX15 Antibody in Western Blot (WB)
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ALOX15 Antibody in Western Blot (WB)
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ALOX15 Antibody (CF504249) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (RC206621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX1. Positive lysates LY400456 (100 µg) and LC400456 (20 µg) can be purchased separately from OriGene. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ALOX15 Antibody in Western Blot (WB)
ALOX15 Monoclonal Antibody (OTI8C5), TrueMAB™

Product Details

CF504249

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI8C5

Immunogen

Full length human recombinant protein of human ALOX15 produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 12-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; 15-LOX; 15-LOX2; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate 15-lipoxygenase; Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; Polyunsaturated fatty acid lipoxygenase ALOX15

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Gene Aliases: 12-LOX; 15-LOX-1; 15LOX-1; ALOX15; LOG15

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UniProt ID: (Human) P16050

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Entrez Gene ID: (Human) 246

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Function(s)
arachidonate 12-lipoxygenase activity iron ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding hepoxilin-epoxide hydrolase activity arachidonate 15-lipoxygenase activity hepoxilin A3 synthase activity eoxin A4 synthase activity
Process(es)
ossification negative regulation of adaptive immune response phosphatidylethanolamine biosynthetic process leukotriene metabolic process inflammatory response positive regulation of cell-substrate adhesion arachidonic acid metabolic process lipoxygenase pathway bone mineralization positive regulation of actin filament polymerization positive regulation of heterotypic cell-cell adhesion response to endoplasmic reticulum stress regulation of peroxisome proliferator activated receptor signaling pathway cellular response to interleukin-13 wound healing apoptotic cell clearance hepoxilin biosynthetic process oxidation-reduction process positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion regulation of engulfment of apoptotic cell lipoxin A4 biosynthetic process
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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