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Invitrogen

AJUBA Recombinant Rabbit Monoclonal Antibody (8P3P8)

View all (10) AJUBA antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite AJUBA Recombinant Rabbit Monoclonal Antibody (8P3P8)

AJUBA Recombinant Rabbit Monoclonal Antibody (8P3P8)
  • Antibody Testing Data (3)
AJUBA Antibody in Western Blot (WB)
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AJUBA Antibody in Western Blot (WB)
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AJUBA Antibody (MA5-51213) in WB

Western blot analysis of AJUBA in extracts from wild type (WT) and Ajuba knockdown (KD) HepG2 cells. Samples were incubated with AJUBA Recombinant monoclonal antibody (Product # MA5-51213) using a dilution of 1:20,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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AJUBA Antibody in Western Blot (WB)
AJUBA Antibody in Western Blot (WB)
AJUBA Antibody in Immunoprecipitation (IP)

Product Details

MA5-51213

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:20,000
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8P3P8

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-290 of human Ajuba (NP_116265.1).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3093850

Product Specific Information

Immunogen sequence: PSAYPELHAA LDRLYAQRPA GFGCQESRHS YPPALGSPGA LAGAGVGAAG PLERRGAQPG RHSVTGYGDC AVGARYQDEL TALLRLTVGT

Target Information

AJUBA (Ajuba LIM Protein) is a Protein Coding gene. Diseases associated with AJUBA include Wilson-Turner X-Linked Mental Retardation Syndrome and Syndromic X-Linked Intellectual Disability. Among its related pathways are Hippo signaling pathway and Adhesion. Gene Ontology (GO) annotations related to this gene include chromatin binding and actin filament binding. An important paralog of this gene is LIMD1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ajuba homolog; Ajuba protein; jub, ajuba homolog; LIM domain-containing protein ajuba; protein ajuba

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Gene Aliases: AJUBA; JUB

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UniProt ID: (Human) Q96IF1, (Rat) Q5U2Z2

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Entrez Gene ID: (Human) 84962, (Rat) 85265

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Function(s)
chromatin binding transcription corepressor activity protein binding zinc ion binding alpha-catenin binding actin filament binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to hypoxia transcription, DNA-templated calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules lamellipodium assembly regulation of cell migration positive regulation of cellular biosynthetic process positive regulation of protein complex assembly negative regulation of kinase activity positive regulation of kinase activity cellular protein localization gene silencing by miRNA wound healing, spreading of epidermal cells negative regulation of hippo signaling regulation of GTPase activity positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity glycerophospholipid biosynthetic process focal adhesion assembly regulation of cellular response to hypoxia negative regulation of nucleic acid-templated transcription positive regulation of gene silencing by miRNA G2/M transition of mitotic cell cycle
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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