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  • AGK Antibodies

Invitrogen

AGK Polyclonal Antibody

View all (11) AGK antibodies

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Questions & Answers

Cite AGK Polyclonal Antibody

  • Antibody Testing Data (1)
AGK Antibody in Western Blot (WB)
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AGK Antibody in Western Blot (WB)
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AGK Antibody (PA5-97316) in WB

Western blot analysis of AGK in SK-MEL-28 cells. Samples were incubated with AGK Polyclonal antibody (Product # PA5-97316) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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AGK Antibody in Western Blot (WB)
AGK Polyclonal Antibody

Product Details

PA5-97316

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 233-422 of human AGK (NP_0607081)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2809118

Product Specific Information

Immunogen sequence: KAAHFFSTLK EWPQTHQASI SYTGPTERPP NEPEETPVQR PSLYRRILRR LASYWAQPQD ALSQEVSPEV WKDVQLSTIE LSITTRNNQL DPTSKEDFLN ICIEPDTISK GDFITIGSRK VRNPKLHVEG TECLQASQCT LLIPEGAGGS FSIDSEEYEA MPVEVKLLPR KLQFFCDPRK REQMLTSPTQ; Positive Samples: 293T, SH-SY5Y, HepG2, HeLa, 22Rv1, BT-474, K-562, Rat brain; Cellular Location: Mitochondrion membrane

Target Information

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acylglycerol kinase, mitochondrial; FLJ10842; hAGK; HsMuLK; multi-substrate lipid kinase; Multiple substrate lipid kinase

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Gene Aliases: AGK; CATC5; CTRCT38; MTDPS10; MULK; RGD1562046

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UniProt ID: (Human) Q53H12

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Entrez Gene ID: (Human) 55750, (Rat) 502749

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Function(s)
ceramide kinase activity diacylglycerol kinase activity ATP binding acylglycerol kinase activity lipid kinase activity NAD+ kinase activity
Process(es)
protein kinase C-activating G-protein coupled receptor signaling pathway ceramide biosynthetic process lipid phosphorylation long-chain fatty-acyl-CoA biosynthetic process glycerolipid metabolic process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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