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  • ADK Antibodies

Invitrogen

ADK Monoclonal Antibody (7F4)

View all (19) ADK antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ADK Monoclonal Antibody (7F4)

  • Antibody Testing Data (6)
ADK Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ADK Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ADK Antibody (MA5-49220) in IHC (P)

Immunohistochemistry analysis of ADK in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with ADK Monoclonal antibody (Product # MA5-49220) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-mouse IgG was used ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ADK Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ADK Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ADK Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ADK Antibody in Western Blot (WB)
ADK Antibody in Flow Cytometry (Flow)
ADK Antibody in Flow Cytometry (Flow)
ADK Monoclonal Antibody (7F4)

Product Details

MA5-49220

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7F4

Immunogen

E. coli-derived human ADK recombinant protein (Position: K165-T351).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074878

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Human ADK shares 88.8% and 88.2% amino acid (aa) sequence identity with mouse and rat ADK, respectively.

Positive Control - WB: human PC-3 whole cell, human U-87MG whole cell, human SW620 whole cell. IHC: human placenta tissue, human tonsil tissue, human rectal cancer tissue. Flow: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Adenosine 5'-phosphotransferase; Adenosine kinase; AK; testicular tissue protein Li 14

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Gene Aliases: ADK; AK

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UniProt ID: (Human) P55263

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Entrez Gene ID: (Human) 132

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Function(s)
adenosine kinase activity ATP binding poly(A) RNA binding metal ion binding
Process(es)
dATP biosynthetic process ribonucleoside monophosphate biosynthetic process positive regulation of cardiac muscle hypertrophy phosphorylation circadian regulation of gene expression positive regulation of T cell proliferation purine-containing compound salvage AMP salvage type B pancreatic cell proliferation adenosine metabolic process
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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