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  • Primary Antibodies ›
  • ACSL3 Antibodies

Invitrogen

ACSL3 Polyclonal Antibody

1 Published Figure
1 Reference
View all (13) ACSL3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ACSL3 Polyclonal Antibody

  • Antibody Testing Data (4)
  • Published Figures (1)
ACSL3 Antibody in Immunocytochemistry (ICC/IF)
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ACSL3 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 5

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ACSL3 Antibody (PA5-29507) in ICC/IF

ACSL3 Polyclonal Antibody detects ACSL3 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACSL3 stained by ACSL3 Polyclonal Antibody (Product # PA5-29507) diluted at 1:500. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ACSL3 Antibody in Immunocytochemistry (ICC/IF)
ACSL3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ACSL3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ACSL3 Antibody in Western Blot (WB)
ACSL3 Antibody in Immunohistochemistry (IHC)

Product Details

PA5-29507

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACSL3. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546983

Product Specific Information

Recommended positive controls: 293T, A431, Jurkat, Raji.

Predicted reactivity: Mouse (95%), Rat (95%), Pig (95%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

An initial reaction in fatty acid metabolism in eukaryotic cells is activation of fatty acids catalyzed by acyl-CoA synthetase. FACL3 (fatty acid CoA ligase, long-chain 3) is identified as member of the acyl-CoA synthetase (ACS) family by PCR of rat brain cDNAs using primers based on the conserved region of the ACS protein. The 720-amino acid rat protein preferentially utilizes myristate, laurate, arachidonate, and eicosapentaenoate, and is expressed primarily in brain. The predicted 720-amino acid FACL3 human protein is 92% identical to that of rat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Arachidonate--CoA ligase; Fatty acid CoA ligase Acsl3; fatty-acid-Coenzyme A ligase, long-chain 3; LACS 3; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 3; Long-chain-fatty-acid--CoA ligase 3; Medium-chain acyl-CoA ligase Acsl3

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Gene Aliases: ACS3; ACSL3; FACL3; LACS3; PRO2194

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UniProt ID: (Human) O95573

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Entrez Gene ID: (Human) 2181

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Function(s)
long-chain fatty acid-CoA ligase activity ATP binding protein kinase binding protein domain specific binding decanoate--CoA ligase activity
Process(es)
long-chain fatty acid metabolic process fatty acid biosynthetic process brain development response to nutrient response to organic cyclic compound very-low-density lipoprotein particle assembly long-chain fatty-acyl-CoA biosynthetic process positive regulation of Golgi to plasma membrane protein transport long-chain fatty acid import positive regulation of secretion positive regulation of phosphatidylcholine biosynthetic process
It has to be done as per old AB suggested Products section.
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