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  • Primary Antibodies ›
  • ACOT9 Antibodies

Invitrogen

ACOT9 Polyclonal Antibody

View all (14) ACOT9 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite ACOT9 Polyclonal Antibody

  • Antibody Testing Data (3)
ACOT9 Antibody in Immunocytochemistry (ICC/IF)
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ACOT9 Antibody in Immunocytochemistry (ICC/IF)
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ACOT9 Antibody (PA5-103565) in ICC/IF

Immunocytochemistry analysis of ACOT9 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ACOT9 polyclonal antibody (Product # PA5-103565) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ACOT9 Antibody in Immunocytochemistry (ICC/IF)
ACOT9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ACOT9 Antibody in Western Blot (WB)
ACOT9 Polyclonal Antibody

Product Details

PA5-103565

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ACOT9(Accession Q9Y305), corresponding to amino acid residues S136-F186.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2852901

Product Specific Information

Antibody detects endogenous levels of total ACOT9.

Target Information

Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT9 (acyl-CoA thioesterase 9), also known as ACATE2, MT-ACT48 (mitochondrial acyl-CoA thioesterase of 48 kDa) or CGI-16, is a 406 amino acid member of the acyl-CoA hydrolase protein family. ACOT9 contains a C-terminal 80-amino acid domain that is conserved from mouse to human, suggesting that the C-terminus may confer the catalytic activity of ACOT9. The gene encoding ACOT9 is located on chromosome X and the expressed ACOT9 protein is localized to the mitochondrion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acyl coenzyme A thioester hydrolase 2; Acyl-CoA thioester hydrolase 9; Acyl-CoA thioesterase 9; acyl-Coenzyme A thioesterase 2, mitochondrial; Acyl-coenzyme A thioesterase 9, mitochondrial; Mitochondrial 48 kDa acyl-CoA thioester hydrolase 1; mitochondrial Acyl-CoA Thioesterase; MT-ACT48.1; MTE-2; p48; Protein U8

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Gene Aliases: 0610041P13Rik; ACATE2; ACOT9; C76421; CGI-16; MT-ACT48; MTACT48; p48; U8

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UniProt ID: (Human) Q9Y305, (Mouse) Q9R0X4

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Entrez Gene ID: (Human) 23597, (Mouse) 56360

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Function(s)
acetyl-CoA hydrolase activity acyl-CoA hydrolase activity carboxylic ester hydrolase activity protein binding CoA hydrolase activity palmitoyl-CoA hydrolase activity hydrolase activity 2-oxoglutaryl-CoA thioesterase activity 2,4,4-trimethyl-3-oxopentanoyl-CoA thioesterase activity 3-isopropylbut-3-enoyl-CoA thioesterase activity glutaryl-CoA hydrolase activity
Process(es)
acyl-CoA metabolic process long-chain fatty-acyl-CoA biosynthetic process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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