Invitrogen

ACADVL Polyclonal Antibody

Name: ACADVL Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-78710

View all (12) ACADVL antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)

ACADVL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

ACADVL Antibody (PA5-78710) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of ACADVL in U2OS cells using ACADVL Polyclonal Antibody (Product # PA5-78710). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter s... View More

ACADVL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ACADVL Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image ACADVL Polyclonal Antibody

Product Details

PA5-78710

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

Immunohistochemistry (IHC)

2-5 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ACADVL (538-576aa RALEQFATVVEAKLIKHKKGIVNEQFLLQRLADGAIDLY).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2745826

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell lysates, human SiHa whole cell lysates, human A431 whole cell lysates, human U251 whole cell lysates, rat liver tissue lysates, rat heart tissue lysates, mouse liver tissue lysates, mouse heart tissue lysates. IHC: human liver cancer tissue, human ovarian cancer tissue, rat heart tissue. Flow: U251 cell.

Target Information

The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acyl-Coenzyme A dehydrogenase, very long chain; MVLCAD; Very long chain Acyl-Coa dehydrogenase; Very long-chain specific acyl-CoA dehydrogenase, mitochondrial; VLCAD; VLCAD very-long-chain acyl-CoA dehydrogenase

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Gene Aliases: ACAD6; ACADVL; LCACD; VLCAD

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UniProt ID: (Human) P49748, (Rat) P45953, (Mouse) P50544

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Entrez Gene ID: (Human) 37, (Rat) 25363, (Mouse) 11370

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Function(s)

fatty-acyl-CoA binding acyl-CoA dehydrogenase activity long-chain-acyl-CoA dehydrogenase activity electron carrier activity very-long-chain-acyl-CoA dehydrogenase activity flavin adenine dinucleotide binding oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors

Process(es)

temperature homeostasis fatty acid beta-oxidation energy derivation by oxidation of organic compounds epithelial cell differentiation fatty acid beta-oxidation using acyl-CoA dehydrogenase IRE1-mediated unfolded protein response very long-chain fatty acid catabolic process negative regulation of fatty acid biosynthetic process negative regulation of fatty acid oxidation lipid homeostasis regulation of cholesterol metabolic process lipid metabolic process fatty acid metabolic process metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ACADVL Polyclonal Antibody

ACADVL Antibody (PA5-78710) in ICC/IF

Product Details

PA5-78710

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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