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  • ACADL Antibodies

Invitrogen

ACADL Polyclonal Antibody

View all (11) ACADL antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite ACADL Polyclonal Antibody

  • Antibody Testing Data (3)
ACADL Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ACADL Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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ACADL Antibody (PA5-109332) in IHC (P)

Immunohistochemistry analysis of ACADL in paraffin-embedded human kidney tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with ACADL polyclonal antibody (Product # PA5-109332) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ACADL Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ACADL Antibody in Western Blot (WB)
ACADL Antibody in Flow Cytometry (Flow)
ACADL Polyclonal Antibody

Product Details

PA5-109332

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:1,000
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within Human ACADL aa 218-430
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2854743

Product Specific Information

Positive Control: SH-SY5Y cell, human kidney tissue, F9.

Target Information

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acyl-Coenzyme A dehydrogenase, long chain; LCAD; Long-chain specific acyl-CoA dehydrogenase, mitochondrial

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Gene Aliases: ACAD4; ACADL; LCAD

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UniProt ID: (Human) P28330

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Entrez Gene ID: (Human) 33

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Function(s)
fatty-acyl-CoA binding acyl-CoA dehydrogenase activity long-chain-acyl-CoA dehydrogenase activity electron carrier activity palmitoyl-CoA oxidase activity flavin adenine dinucleotide binding oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
Process(es)
temperature homeostasis fatty acid beta-oxidation carnitine metabolic process, CoA-linked fatty acid beta-oxidation using acyl-CoA dehydrogenase carnitine catabolic process long-chain fatty acid catabolic process cellular lipid catabolic process negative regulation of fatty acid biosynthetic process negative regulation of fatty acid oxidation protein homotetramerization lipid homeostasis oxidation-reduction process regulation of cholesterol metabolic process
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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