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  • ABI2 Antibodies

Bethyl Laboratories

ABI2 Polyclonal Antibody

View all (10) ABI2 antibodies

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Datasheet
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Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ABI2 Polyclonal Antibody

  • Antibody Testing Data (1)
ABI2 Antibody in Western Blot (WB)
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ABI2 Antibody in Western Blot (WB)
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ABI2 Antibody (A302-499A) in WB

Detection of human ABI2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ABI2 antibody (Product # A302-499A) used for WB at 0.4 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. ABI2 was also immunoprecipitated by rabbit anti-ABI2 antibody BL9105, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ABI2 Antibody in Western Blot (WB)
ABI2 Polyclonal Antibody

Product Details

A302-499A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunoprecipitation (IP)

2-5 µg/mg lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 351 and 401 of human abl interactor 2.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

ABI2 may act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. ABI2 is a part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. It regulates ABL1/c-Abl-mediated phosphorylation of MENA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Abelson interactor 2; Abi-2; abl binding protein 3; Abl interactor 2; Abl-binding protein 3; abl-interacting protein 1 (SH3-containing protein); abl-interactor protein 2b; AblBP3; arg protein tyrosine kinase-binding protein; Arg-binding protein 1; ArgBP1

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Gene Aliases: ABI-2; ABI2; ABI2B; AblBP3; AIP-1; argBP1; ARGBPIA; argBPIB; SSH3BP2

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UniProt ID: (Human) Q9NYB9

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Entrez Gene ID: (Human) 10152

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Function(s)
DNA binding protein binding cytoskeletal adaptor activity SH3 domain binding kinase binding ubiquitin protein ligase binding protein complex binding Rac GTPase binding proline-rich region binding
Process(es)
movement of cell or subcellular component cytoskeleton organization actin polymerization or depolymerization viral process cell migration Rac protein signal transduction peptidyl-tyrosine phosphorylation positive regulation of Arp2/3 complex-mediated actin nucleation
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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