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  • 14-3-3 theta Antibodies

Invitrogen

14-3-3 theta Polyclonal Antibody

Advanced Verification
2 Published Figures
1 Reference
View all (21) 14-3-3 theta antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite 14-3-3 theta Polyclonal Antibody

  • Antibody Testing Data (4)
  • Published Figures (2)
  • Advanced Verification (1)
14-3-3 theta Antibody in Immunocytochemistry (ICC/IF)
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14-3-3 theta Antibody in Immunocytochemistry (ICC/IF)
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14-3-3 theta Antibody (PA5-18822) in ICC/IF

Immunofluorescence analysis of 14-3-3 theta was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 14-3-3 theta Goat Polyclonal Antibody (Product # PA5-18822) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labele... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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14-3-3 theta Antibody in Immunocytochemistry (ICC/IF)
14-3-3 theta Antibody in Western Blot (WB)
14-3-3 theta Antibody in Western Blot (WB)
14-3-3 theta Antibody in Western Blot (WB)
14-3-3 theta Antibody in Western Blot (WB)
14-3-3 theta Antibody in Western Blot (WB)
14-3-3 theta Antibody
14-3-3 theta Polyclonal Antibody

Product Details

PA5-18822

Applications
Tested Dilution
Publications

Western Blot (WB)

0.03-0.1 µg/mL
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL
-
Product Specifications

Species Reactivity

Human

Published species

Dog

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DDRKQTIDNSQ) corresponding to the internal amino acids of YWHAQ (aa 136-147).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984084

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 4,000.

Target Information

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; Protein HS1; protein tau; protein, theta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

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Gene Aliases: 14-3-3; 1C5; HS1; YWHAQ

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UniProt ID: (Human) P27348

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Entrez Gene ID: (Human) 10971

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Function(s)
protein binding protein C-terminus binding protein domain specific binding ion channel binding protein N-terminus binding 14-3-3 protein binding
Process(es)
protein targeting small GTPase mediated signal transduction substantia nigra development negative regulation of ion transmembrane transport negative regulation of transcription, DNA-templated membrane organization positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
It has to be done as per old AB suggested Products section.
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