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  • 14-3-3 theta Antibodies

Invitrogen

14-3-3 theta Polyclonal Antibody

View all (21) 14-3-3 theta antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite 14-3-3 theta Polyclonal Antibody

  • Antibody Testing Data (1)
14-3-3 theta Antibody in Western Blot (WB)
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14-3-3 theta Antibody in Western Blot (WB)
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14-3-3 theta Antibody (PA5-119511) in WB

Western blot analysis of 14-3-3 theta in various whole cell extracts (30 µg). Samples were incubated with 14-3-3 theta polyclonal antibody (Product # PA5-119511) using a dilution of 1:3,000 followed by HRP-conjugated anti-rabbit IgG. Separated by 12% SDS-PAGE. Competitors antibody diluted at 1:3,000. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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14-3-3 theta Antibody in Western Blot (WB)
14-3-3 theta Polyclonal Antibody

Product Details

PA5-119511

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of rat 14-3-3 theta. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2913085

Product Specific Information

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Information

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; Protein HS1; protein tau; protein, theta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

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Gene Aliases: 14-3-3; 14-3-3t; 1C5; HS1; YWHAQ

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UniProt ID: (Human) P27348, (Rat) P68255

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Entrez Gene ID: (Human) 10971, (Rat) 25577

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Function(s)
protein binding protein C-terminus binding protein domain specific binding ion channel binding protein N-terminus binding 14-3-3 protein binding
Process(es)
protein targeting small GTPase mediated signal transduction substantia nigra development negative regulation of ion transmembrane transport negative regulation of transcription, DNA-templated membrane organization positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
It has to be done as per old AB suggested Products section.
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