Invitrogen

Mouse CD200 His-tag Recombinant Protein

Name: Mouse CD200 His-tag Recombinant Protein
Brand: Invitrogen
SKU: RP-88210

View all (5) CD200 proteins

Datasheet

Tech Support

Datasheet

Tech Support

Testing Data (1)

Mouse CD200 His-tag Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Mouse CD200 His-tag Protein (RP-88210) in SDS-PAGE

SDS-PAGE analysis of Mouse CD200 His-tag (Product # RP-88210) shows purity to be: > 95 % (SDS-PAGE).

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Product Details

RP-88210

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species

Mouse

Expression System

HEK293 cells

Amino acid sequence

Mouse CD200, amino acids Met1-Gly232 (Accession # O54901.1) with a C-terminal His-tag

Tag

His-tag

Molecular weight

24 kDa

Class

Recombinant

Type

Protein

Purity

>95% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD200 at 1 µg/ml (100 µL/well) can bind mouse CD200R1/Fc with a linear range of 1.5-200 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (1786 µL) be added to the vial to prepare a stock solution of 0.56 mg/mL.

Concentration is measured by UV.

Target Information

CD200 is a type-1 membrane glycoprotein in the immunoglobulin superfamily, featuring two immunoglobulin domains. It is widely expressed on various cells, including B and T cells, keratinocytes, nerve cells, follicular dendritic cells, ovarian cells, and trophoblasts. CD200 interacts with CD200R on myeloid cells, inhibiting macrophage activation, reducing IL-6 production, and attenuating lymphocyte activation. It also prevents mast cell degranulation and decreases TNF-alpha and IL-13 secretion, playing a role in immune regulation and maternal tolerance. Homologues of CD200 are found in herpesviruses and poxviruses, indicating a conserved immune modulation function. CD200 has multiple alternatively spliced transcript variants, contributing to its functional diversity. Diseases linked to CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy, highlighting its importance in health and disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody MRC OX-2; MRC OX-2 antigen; OX-2 membrane glycoprotein