Invitrogen

Human S100B His-tag Recombinant Protein

Name: Human S100B His-tag Recombinant Protein
Brand: Invitrogen
SKU: RP-88161

View all (5) S100B proteins

Datasheet

Tech Support

Datasheet

Tech Support

Testing Data (1)

Human S100B His-tag Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Human S100B His-tag Protein (RP-88161) in SDS-PAGE

SDS-PAGE analysis of Human S100B His-tag (Product # RP-88161) shows purity to be: ≥ 90 % (SDS-PAGE), ≥ 90 % (SEC-HPLC).

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Product Details

RP-88161

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human S100B, amino acids Ser2-Glu92 (Accession # NP_006263.1) with an N-terminal His-tag

Tag

His-tag

Molecular weight

12 kDa

Class

Recombinant

Type

Protein

Purity

>90% as determined by SDS-PAGE.

Activity

1. Measured by its ability to bind human AGER in a functional ELISA. 2. Measured by its ability to bind TP53 in a functional ELISA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.5, with 10% glycerol, 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.

Concentration is measured by UV.

Target Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S100 protein beta chain