Gibco

Human FGF-4, Animal-Free Recombinant Protein, PeproTech®

Name: Human FGF-4, Animal-Free Recombinant Protein, PeproTech\u00AE
Brand: Gibco
SKU: AF-100-31-25UG

11 References

View all (6) FGF4 proteins

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

AF-100-31-25UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 10 publications 10 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse, Pig

Expression System

E. coli

Amino acid sequence

GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

Molecular weight

19.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 10^5 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AF-100-31-1MG will be provided as 2 x 500 µg (AF-100-31-500UG).

Recombinant Human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: FGF; FGF-4; Fibroblast growth factor; Fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; oncogene HST; Transforming protein KS3