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  • Proteins & Peptides ›
  • CD154 (CD40 Ligand) Proteins

Gibco

Human CD40 Ligand (TNFSF5) (soluble) Recombinant Protein, PeproTech®

46 References
View all (6) CD154 (CD40 Ligand) proteins

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Datasheet
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Datasheet
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Cite Human CD40 Ligand (TNFSF5) (soluble) Recombinant Protein, PeproTech®

  • Testing Data (1)
Human CD40 Ligand (TNFSF5) (soluble) Protein in Functional Assay (Functional)
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Human CD40 Ligand (TNFSF5) (soluble) Protein in Functional Assay (Functional)
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FIGURE: 1 / 1

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Human CD40 Ligand (TNFSF5) (soluble) Protein (310-02-50UG) in Functional

Bioassay analysis of Human CD40 Ligand (TNFSF5) (soluble) Recombinant Protein, PeproTech® (Product # 310-02-1MG). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human CD40 Ligand (TNFSF5) (soluble) Protein in Functional Assay (Functional)

Product Details

310-02-50UG

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-

Functional Assay (Functional)

Assay-dependent
-

T-Cell Activation (TCA)

-
View 1 publication 1 publication

In vitro Assay (IV)

-
View 43 publications 43 publications

Miscellaneous PubMed (MISC)

-
View 2 publications 2 publications
Product Specifications

Species

Human

Published species

Bacteria, Human, Mouse, Non-human primate, Rhesus monkey

Expression System

E. coli

Amino acid sequence

MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

Molecular weight

16.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5-10 ng/ml. Note: Results may vary with PBMC donors.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD154; CD40 antigen ligand; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

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Gene Aliases: CD154; CD40L; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP

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UniProt ID: (Human) P29965

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Entrez Gene ID: (Human) 959

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It has to be done as per old AB suggested Products section.
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