Invitrogen

Human B7-1 (CD80) His-tag Recombinant Protein

Name: Human B7-1 (CD80) His-tag Recombinant Protein
Brand: Invitrogen
SKU: RP-88024

View all (3) CD80 (B7-1) proteins

Datasheet

Tech Support

Datasheet

Tech Support

Testing Data (1)

Human B7-1 (CD80) His-tag Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Human B7-1 (CD80) His-tag Protein (RP-88024) in SDS-PAGE

SDS-PAGE analysis of Human B7-1 (CD80) His-tag (Product # RP-88024). The specification for purity shows: ≥ 95 % (SDS-PAGE), ≥ 90 % (SEC-HPLC).

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Product Details

RP-88024

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human B7-1, amino acids Met1-Asn242 (Accession # NP_005182.1) with a C-terminal His-tag

Tag

His-tag

Molecular weight

45-48 kDa

Class

Recombinant

Type

Protein

Purity

>95% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant (His Tag) at 2 µg/ml (100 µL/well) can bind CTLA-4 Protein, Human, Recombinant (His and hFc Tag) . The EC50 of of CTLA-4 (His and hFc Tag) is 35-80 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (1886 µL) be added to the vial to prepare a stock solution of 0.53 mg/mL.

Concentration is measured by UV.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activation B7-1 antigen; B-lymphocyte activation antigen B7; B7; BB1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; sCD 80; sCD80; soluble CD 80; soluble CD80; T-lymphocyte activation antigen CD80; TS/A-1