Invitrogen
Vitamin D2/D3 Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Shipping conditions
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Protein name
Species (tested)
Species (reported)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Vitamin D (VD) ELISA quantitates VD in serum, plasma and other biological fluids.
Principle of the method
The Competitive mulispecies ELISA research use only kit is designed to quantitatively measure Vitamin D (VD)independent of species . A Vitamin D standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve.Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Vitamin D is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Vitamin D in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Vitamin D2 and vitamin D3 are two forms of vitamin D, a group of fat-soluble vitamins that play crucial roles in maintaining overall health. Vitamin D2, also known as ergocalciferol, is derived from plant sources. It is produced when ultraviolet (UV) light converts ergosterol, a compound found in certain fungi and plants, into vitamin D2. Vitamin D3, also called cholecalciferol, is synthesized in the skin of humans and animals upon exposure to UVB light from the sun. It is the most biologically active form of vitamin D and is also found in certain animal-based food sources. Both vitamin D2 and D3 undergo similar metabolic processes in the liver and kidneys to be converted into their active form, known as calcitriol. Calcitriol acts as a hormone that regulates calcium and phosphorus metabolism in the body.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases : Cholecalciferol, Ergocalciferol, Vitamin D2, vitamin D3, calciferol
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