Invitrogen
Rat Testosterone Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Shipping conditions
Storage
Protein name
Species (tested)
Species (reported)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Rat Testosterone (T) ELISA quantitates T in serum and plasma.
Principle of the method
The Rat T solid phase competitive ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between the pre coated antigen and the HRP linked antibody specific for Rat T. A target specific antigen has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Rat T in the samples (or standards) competes with the immobilized (capture) antigen for sites on the HRP linked detection antibody. Excess conjugate and unbound sample or standard are washed away from the plate. The substrate solution is added to each well, and the enzyme substrate reaction is terminated by the addition of a stop solution, turning the color yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The intensity of this signal is inversely proportional to the concentration of Rat T present in the original specimen, which is determined by comparing the OD of the samples to the standard curve.
Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases : Androderm, C19H28O2, Mertestate, Sustanon, Testoderm, Testosteron
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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