Invitrogen
Rat IL-13-Cell Lysates ELISA Kit
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Product Specifications
Analytical sensitivity
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Time-to-result
Homogenous (no wash)
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Intraassay CV
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Species (tested)
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Detector antibody conjugate
Label or dye
About This Kit
The Rat Interleukin 13-Cell Lysates (Rt IL-13) ELISA quantitates Rt IL-13 in cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Rt IL-13.
Principle of the method
The Rat IL-13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : Il-13, Il13
Gene ID : (Rat) 116553
Gene symbol : Il13
Protein Aliases : IL-13, T-cell activation protein P600, ALRH, ALRH antibody, BHR1, MGC116786, Interleukin13, il 13, MGC116788, MGC116789, NC30 antibody, P600 antibody, ILN, Interleukin, Interleukin-13
UniProt ID (Rat) P42203
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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