Invitrogen
Rabbit Estradiol Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
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Contents
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Protein name
Species (tested)
Species (reported)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Rabbit Estradiol (E2) ELISA quantitates E2 in serum and plasma.
Principle of the method
The Rabbit E2 solid phase competitive ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between the pre coated antigen and the HRP linked antibody specific for Rabbit E2. A target specific antigen has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Rabbit E2 in the samples (or standards) competes with the immobilized (capture) antigen for sites on the HRP linked detection antibody. Excess conjugate and unbound sample or standard are washed away from the plate. The substrate solution is added to each well, and the enzyme substrate reaction is terminated by the addition of a stop solution, turning the color yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The intensity of this signal is inversely proportional to the concentration of Rabbit E2 present in the original specimen, which is determined by comparing the OD of the samples to the standard curve.
Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Estradiol (17 beta-estradiol) is a sex hormone. Estradiol is the predominant sex hormone present in females. It is also present in males, being produced as an active metabolic product of testosterone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases : 17-Beta-Estradiol, 17-beta-HSD
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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