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Invitrogen

Multispecies JNK1/JNK2 (Total/Phospho) InstantOne™ ELISA Kit

View all (3) JNK1/JNK2 ELISA Kits

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Technical guide & protocol Tech Support
Multispecies JNK1/JNK2 (Total/Phospho) InstantOne™ ELISA Kit (85-86198-11)
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Multispecies JNK1/JNK2 (Total/Phospho) InstantOne™ ELISA Kit (85-86198-11)
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Multispecies JNK1/JNK2 (Total/Phospho) InstantOne™ ELISA Kit

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Multispecies JNK1/JNK2 (Total/Phospho) InstantOne™ ELISA Kit (85-86198-11)
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Product Specifications

Sample type/volume

Cell Lysate
20 µL

Hands-on time

1 hr 20 min

Time-to-result

1 hr 10 min

Homogenous (no wash)

No

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

JNK1/JNK2

Target sub-specificity

Total

Species (tested)

Human, Mouse

Assay kit format

InstantOne ELISA

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse JNK 1/2 in cell lysates. Detection of rat JNK 1/2 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Target information

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : AI849689, AI851083, JNK, JNK-46, JNK-55, JNK1, JNK1A2, JNK2, JNK21B1/2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, MAPK8, MAPK9, p54a, p54aSAPK, PRKM8, PRKM9, SAPK, SAPK1, SAPK1A, SAPK1C

Gene ID : (Human) 5599, (Human) 5601, (Mouse) 26419, (Mouse) 26420

Gene symbol : MAPK8, MAPK9, Mapk8, Mapk9

Protein Aliases : c-Jun kinase 2, C-jun kinase, JNK 55, MAPK, OTTHUMP00000019552, Phospho-JNK, PRKM, protein kinase JNK1, SAPK 1, c-Jun N-terminal kinase 1, c-Jun N-terminal kinase 2, JNK-46, JNK-55, JNK/SAPK alpha, JNK1 beta1 protein kinase, Jun kinase, JUN N-terminal kinase, MAP kinase 8, MAP kinase 9, MAPK 8, MAPK 9, mitogen activated protein kinase 8, mitogen activated protein kinase 9, Mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, Mitogen-activated protein kinase 9, protein kinase mitogen-activated 8, protein kinase, mitogen-activated 9, SAPK1a, SAPK1c, stress-activated protein kinase 1, Stress-activated protein kinase 1a, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, Stress-activated protein kinase JNK2

UniProt ID (Human) P45983, (Human) P45984, (Mouse) Q91Y86, (Mouse) Q9WTU6

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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