Invitrogen
Multispecies AKT/ERK/p70 S6K Activation InstantOne™ ELISA Kit
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Multispecies AKT/ERK/p70 S6K Activation InstantOne™ ELISA Kit
Product Specifications
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Instrument
Product size
Contents
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Storage
Protein name
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), AKT 1/2/3 (human, mouse, hamster), and p70 S6K (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Extracellular signal-regulated kinase (ERK) is the founding member and a key component of the classical Mitogen-Activated Protein Kinase (MAPK) pathway. MEK activates ERK 1/2 by phosphorylation of its TxY motifs in response to a variety of stimuli, including growth factors and cytokines. AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. p70 S6 Kinase (p70S6K) is a member of the ribosomal S6 kinase family of Ser/Thr kinases. p70 S6K activity is controlled by multiple signaling pathways, including the MAPK, phosphoinositide-3 kinase (PI3K), and mTOR pathways. This regulation results in the phosphorylation of multiple sites of p70 S6K, including Thr389 within the linker domain, which is required for full activation. Activated p70 S6K phosphorylates several residues on the S6 ribosomal protein, thereby leading to increased protein synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : AKT, AKT1, AKT2, AKT3, CWS6, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, HIHGHH, HS44KDAP, HUMKER1A, MAPK1, MAPK2, MAPK3, MPPH, MPPH2, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, p70 S6KA, p70(S6K)-alpha, p70-alpha, p70-S6K, PKB, PKB-ALPHA, PKB-GAMMA, PKBB, PKBBETA, PKBG, PRKBA, PRKBB, PRKBG, PRKM1, PRKM2, PRKM3, PS6K, RAC, RAC-ALPHA, RAC-BETA, RAC-gamma, RAC-PK-gamma, RPS6KB1, S6K, S6K-beta-1, S6K1, STK-2, STK14A
Gene ID : (Human) 207, (Human) 208, (Human) 10000, (Human) 5594, (Human) 5595, (Human) 6198
Gene symbol : AKT1, AKT2, AKT3, MAPK1, MAPK3, RPS6KB1
Protein Aliases : 70 kDa ribosomal protein S6 kinase 1, AKT1m, ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, murine thymoma viral (v-akt) homolog-2, p42-MAPK, p44-ERK1, p44-MAPK, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6 kinase, alpha, P70S6K1, PKB, PKB alpha, PKB beta, PKB gamma, Protein kinase Akt-2, Protein kinase Akt-3, Protein kinase B, Protein kinase B alpha, Protein kinase B beta, Protein kinase B gamma, protein tyrosine kinase ERK2, Proto-oncogene c-Akt, putative v-akt murine thymoma viral oncoprotein 2, rac protein kinase alpha, RAC protein kinase beta, RAC-alpha serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-gamma serine/threonine protein kinase, RAC-gamma serine/threonine-protein kinase, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K-beta-1, serine-threonine protein kinase, serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A, STK-2, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene homolog 2, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), v-akt murine thymoma viral oncogene-like protein 1
UniProt ID (Human) P31749, (Human) P31751, (Human) Q9Y243, (Human) P28482, (Human) P27361, (Human) P23443
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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