Invitrogen
Mouse VEGF Receptor 2 (VEGF R2) ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
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Time-to-result
Homogenous (no wash)
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Intraassay CV
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Protein name
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Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Mouse Vascular Endothelial Cell Growth Factor Receptor 2 (VEGF R2) ELISA quantitates Ms VEGF R2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms VEGF R2.
Principle of the method
The Mouse VEGF R2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : 6130401C07, Flk-1, Flk1, Kdr, Krd-1, Ly73, sVEGFR-2, VEGFR-2, VEGFR2
Gene ID : (Mouse) 16542
Gene symbol : Kdr
Protein Aliases : CD309, CD309 antigen, VEGF R2, sCD309, soluble CD309, soluble VEGFR 2, Fetal liver kinase 1, FLK-1, Kinase NYK, Protein-tyrosine kinase receptor flk-1, soluble vascular endothelial growth factor receptor 2, Vascular endothelial growth factor receptor 2, vascular endothelial growth factor receptor- 2, vascular endothelial growth factor receptor-2, vascular endothelial growth factor receptor-3, VEGF receptor-2, VEGFR-2
UniProt ID (Mouse) P35918
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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