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Mouse ST2 (IL-33R) Uncoated ELISA Kit with Plates

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Technical guide & protocol Tech Support
Mouse ST2 (IL-33R) Uncoated ELISA Kit with Plates (88-9334-22)
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Mouse ST2 (IL-33R) Uncoated ELISA Kit with Plates (88-9334-22)
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Mouse ST2 (IL-33R) Uncoated ELISA Kit with Plates

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Mouse ST2 (IL-33R) Uncoated ELISA Kit with Plates (88-9334-22)
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Product Specifications

Analytical sensitivity

80 pg/mL

Assay range

80-10,000 pg/mL

Sample type/volume

Serum
50 µL
Supernatant
50 µL

Hands-on time

1 hr 45 min

Time-to-result

24 hr 30 min

Homogenous (no wash)

No

Instrument

Absorbance Microplate Reader

Product size

2 x 96 Tests

Contents

Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

ST2

Species (tested)

Mouse

Assay kit format

Uncoated ELISA Kit with Plates

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse ST-2 (IL-33R) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-33R. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

Principle of the method

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Target information

IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L - the membrane bound form, and ST2S - the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : DER4, Fit-1, Il1rl1, Ly84, St2, St2-rs1, ST2L, Ste2, T1, T1/ST2

Gene ID : (Mouse) 17082

Gene symbol : Il1rl1

Protein Aliases : Interleukin-1 receptor-like 1, Interleukin-33 receptor alpha chain, Lymphocyte antigen 84, Protein ST2, Protein T1, IL-1 R4, IL-1RL1, IL-33R, Interleukin1 receptor like 1, sIL 33R, soluble IL 33 Receptor, soluble IL 33R

UniProt ID (Mouse) P14719

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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