Invitrogen
Mouse/Rat Clusterin (CLU) ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Storage
Protein name
Protein family
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Rat Clusterin (Rt CLU) ELISA quantitates Rt Clusterin in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Clusterin.
Principle of the method
The Rat Clusterin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : AI893575, APOJ, CLI, Clu, D14Ucla3, DAG, Msgp-2, RATTRPM2B, SGP-2, SGP2, SP-40, SP40, Sugp-2, TRPM-2, Trpm2, TRPM2B, Trpmb
Gene ID : (Rat) 24854, (Mouse) 12759
Gene symbol : Clu, Clu
Protein Aliases : Apo-J, Apolipoprotein J, Clusterin, Clustrin, complement lysis inhibitor, DAG, Dimeric acid glycoprotein, HGNC:2095, MGC24903, Clusterin alpha / beta chain, Complement associated protein SP40, GP80, Ku70-binding protein 1 (KUB1), Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B), ApoJ β, ApoJa, ApoJb, apo j, Aging associated protein 4, Apo Jb, ApoJ a, ApoJ alpha, ApoJ b, ApoJ beta, ApoJ α, Apo J alpha, Apo J b, Apo J beta, Apo J α, Apo J β, Apo Ja, Apo J a, SGP-2, Sulfated glycoprotein 2, testosterone repressed prostate message, Testosterone repressed prostate message 2, testosterone repressed prostate message-2, testosterone-repressed prostate message, testostrone-repressed prostate message 2, TRPM-2
UniProt ID (Rat) P05371, (Mouse) Q06890
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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