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Invitrogen

Mouse NT-proBNP ELISA Kit

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Mouse NT-proBNP ELISA Kit
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Mouse NT-proBNP ELISA Kit
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Mouse NT-proBNP ELISA Kit

ELISA Standard Curve of NT-proBNP using Mouse NT-proBNP ELISA Kit (Product # EEL193). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse NT-proBNP ELISA Kit
Mouse NT-proBNP ELISA Kit (EEL193)
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Product Specifications

Analytical sensitivity

9.38 pg/mL

Assay range

15.63-1,000 pg/mL

Sample type/volume

Cell Lysate
100 µL
Serum
100 µL
Plasma
100 µL
Tissue Homogenate
100 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

NT-proBNP

Species (tested)

Mouse

Species (reported)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse N terminal pro Brain Natriuretic Peptide (NT proBNP) ELISA quantitates NT proBNP in serum, plasma and other biological fluids.

Principle of the method

The Mouse N terminal pro Brain Natriuretic Peptide (NT proBNP) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Target information

The N-terminal prohormone of brain natriuretic peptide is a prohormone with a 76 amino acid N-terminal inactive protein that is cleaved from the molecule to release brain natriuretic peptide 32.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : AA408272, BNF, BNP, Nppb

Gene ID : (Mouse) 18158

Gene symbol : Nppb

Protein Aliases : Brain natriuretic factor prohormone, brain natriuretic peptide, Gamma-brain natriuretic peptide, Iso-ANP, N-terminal pro b-type natriuretic peptide, NTproBNP, natriuretic peptide precursor type B, Natriuretic peptides B, preproBNP, type-B natriuretic factor

UniProt ID (Mouse) P40753

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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