Invitrogen
Mouse KC/CXCL1 ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
Product size
Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Storage
Protein name
Protein family
Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Mouse KC/CXCL1 ELISA quantitates Ms KC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms KC.
Principle of the method
The Mouse KC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : Cxcl1, Fsp, Gro, Gro1, KC, Mgsa, N51, Scyb1
Gene ID : (Mouse) 14825
Gene symbol : Cxcl1
Protein Aliases : alpha-chemokine, C-X-C motif chemokine 1, CXCL-1, C Cmotif chemokine, C X C motif chemokine, CC motif chemokine, GROalpha, GROα, NAP3, CCmotif chemokine, CXC, CXC motif chemokine, CXCL, GRO a, GRO α, GRO1 oncogene, Growth-regulated alpha protein, KC/GR)-alpha, KC/GRO-alpha, Platelet-derived growth factor-inducible protein KC, Secretory protein N51
UniProt ID (Mouse) P12850
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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