Invitrogen
Mouse IL-3 ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
Hands-on time
Time-to-result
Homogenous (no wash)
Interassay CV
Intraassay CV
Instrument
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
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Protein name
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Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Mouse Interleukin-3 (Ms IL-3) ELISA quantitates Ms IL-3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-3.
Principle of the method
The Mouse IL-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : BPA, Csfmu, HCGF, Il-3, Il3, MCGF, PSF
Gene ID : (Mouse) 16187
Gene symbol : Il3
Protein Aliases : BP/BPA, Eo-CSF, MGC79398, MGC79399, MGF, PCSA, Multi CSF, Multilineage colony stimulating factor, multiple), Colony stimulating factor multiple, P cell stimulating factor, ILN, Interleukin, Interleukin3, Interleukin 3 (colony stimulating factor, Hematopoietic growth factor, IL-3, Interleukin-3, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor
UniProt ID (Mouse) P01586
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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