Invitrogen
Mouse ICAM-1 ELISA Kit
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Product Specifications
Analytical sensitivity
Assay range
Sample type/volume
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Time-to-result
Homogenous (no wash)
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Intraassay CV
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Contents
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
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Protein name
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Species (tested)
Assay kit format
Detector antibody conjugate
Label or dye
About This Kit
The Mouse Intercellular Adhesion Molecule 1 (Ms ICAM-1) ELISA quantitates Ms ICAM-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms ICAM-1.
Principle of the method
The Mouse ICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Gene aliases : CD54, Icam-1, Icam1, Ly-47, MALA-2
Gene ID : (Mouse) 15894
Gene symbol : Icam1
Protein Aliases : CD54, ICAM-1, MALA-2, MyD10, sCD54, soluble CD54, Human rhinovirus receptor, Ly 47, MALA2, Surface antigen of activated B cells, Intercellular adhesion molecule 1
UniProt ID (Mouse) P13597
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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