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Invitrogen

Mouse Fc gamma R2B (FCGR2B) ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Mouse Fc gamma R2B (FCGR2B) ELISA Kit
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Mouse Fc gamma R2B (FCGR2B) ELISA Kit
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Mouse Fc gamma R2B (FCGR2B) ELISA Kit

ELISA Standard curve of Fc gamma R2B (FCGR2B) using Mouse Fc gamma R2B (FCGR2B) ELISA Kit (Product # EMFCGR2B). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse Fc gamma R2B (FCGR2B) ELISA Kit
Mouse Fc gamma R2B (FCGR2B) ELISA Kit (EMFCGR2B)
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Product Specifications

Analytical sensitivity

80 pg/mL

Assay range

0.096-70 ng/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

CD32b (FCGR2B)

Protein subtype

Adaptor Proteins

Protein family

Other Proteins

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse Fc gamma R2B (Ms FCGR2B) ELISA quantitates Ms FCGR2B in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms FCGR2B.

Principle of the method

The Mouse FCGR2b solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : AI528646, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr-2, Fcr-3, fcRII, Ly-17, Ly-m20, LyM-1

Gene ID : (Mouse) 14130

Gene symbol : Fcgr2b

Protein Aliases : CD32, CD32 receptor 2, CD32 receptor 2a, CD32 receptor 2b, CD32A, fc-gamma RII-a, fc-gamma-RIIa, FcGR, FCGR2A1, fcRII-a, FGFR2B, Fc gamma receptor IIB, Fc gamma RIIB, Fc-gamma RII, Fc-gamma-RII, fc-gamma-RIIB, FcgammaRIIB, IgG Fc receptor II beta, Low affinity immunoglobulin gamma Fc region receptor II, low affinity immunoglobulin gamma Fc region receptor II-b, Ly-17, Lymphocyte antigen 17

UniProt ID (Mouse) P08101

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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