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Invitrogen

Mouse Eotaxin-2 (CCL24) ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Mouse Eotaxin-2 (CCL24) ELISA Kit
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Mouse Eotaxin-2 (CCL24) ELISA Kit
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Mouse Eotaxin-2 (CCL24) ELISA Kit

ELISA Standard curve of Eotaxin-2 (CCL24) using Mouse Eotaxin-2 (CCL24) ELISA Kit (Product # EMCCL24). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse Eotaxin-2 (CCL24) ELISA Kit
Mouse Eotaxin-2 (CCL24) ELISA Kit (EMCCL24)
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Product Specifications

Analytical sensitivity

4 pg/mL

Assay range

3.28-800 pg/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

Eotaxin 2

Protein family

Cytokines & Receptors

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse Eotaxin-2 (CCL24) ELISA quantitates Ms Eotaxin-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Eotaxin-2.

Principle of the method

The Mouse Eotaxin-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Ccl24, CKb-6, MPIF-2, Scya24

Gene ID : (Mouse) 56221

Gene symbol : Ccl24

Protein Aliases : C-C motif chemokine 24, CC chemokine CCL24, Eosinophil chemotactic protein 2, Eotaxin-2, Small-inducible cytokine A24, Eotaxin2, small inducible cytokine A24

UniProt ID (Mouse) Q9JKC0

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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