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Invitrogen

Mouse Endocan (ESM1) ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Mouse Endocan (ESM1) ELISA Kit
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Mouse Endocan (ESM1) ELISA Kit
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Mouse Endocan (ESM1) ELISA Kit

ELISA Standard curve of Endocan (ESM1) using Mouse Endocan (ESM1) ELISA Kit (Product # EMESM1). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse Endocan (ESM1) ELISA Kit
Mouse Endocan (ESM1) ELISA Kit (EMESM1)
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Product Specifications

Analytical sensitivity

0.1 ng/mL

Assay range

0.123-30 ng/mL

Sample type/volume

Plasma
50 µL
Serum
50 µL
Supernatant
100 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Protein name

Endocan

Protein family

Growth Factors & Receptors

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse Endocan (Ms ESM1) ELISA quantitates Ms Endocan in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Endocan.

Principle of the method

The Mouse Endocan solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Endothelial cell-specific molecule 1 is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : 0610042H23Rik, AV004503, ESM-1, Esm1

Gene ID : (Mouse) 71690

Gene symbol : Esm1

Protein Aliases : Endothelial cell-specific molecule 1, ESM-1, IGFBP-rp6

UniProt ID (Mouse) Q9QYY7

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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