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Invitrogen

Mouse C-Peptide ELISA Kit

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Technical guide & protocol Tech Support
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Technical guide & protocol Tech Support
Mouse C-Peptide ELISA Kit
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Mouse C-Peptide ELISA Kit
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Mouse C-Peptide ELISA Kit

ELISA Standard Curve of C-Peptide using Mouse C-Peptide ELISA Kit (Product # EEL093). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse C-Peptide ELISA Kit
Mouse C-Peptide ELISA Kit (EEL093)
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Product Specifications

Analytical sensitivity

0.1 ng/mL

Assay range

0.16-10 ng/mL

Sample type/volume

Serum
100 µL
Plasma
100 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

C-Peptide

Protein family

Insulin

Species (tested)

Mouse

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Mouse C-Peptide ELISA quantitates C-Peptide in serum, plasma, and other biological fluids

The Mouse C-Peptide solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Ins-1, Ins1, Ins2-rs1

Gene ID : (Mouse) 16333

Gene symbol : Ins1

Protein Aliases : Cpeptide, Insulin-1

UniProt ID (Mouse) P01325

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SIZE

10 x 96 tests

PRICE

USD 400.00

Cat # CHC1263

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Performance Guarantee

If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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